T Cell Receptor and the B Cell Receptor: Comparison

T Cell Receptor and the B Cell Receptor: Comparison The entire world is full of pathogens which we need to fight off to leave a normal life. Due to this, we have an immune system that helps us fight off and prevent/manage subsequent infections. Our immune system can be classified into two, the innate and acquired immune responses. The innate immune response is broadly specific and provides the first defensive action against any infection. Their response to any subsequent infection stays the same as the initial infection. In contrast, the acquired immune response is highly specific such that it provides defence by generating antibodies specific to an antigen. They also have the capacity of keeping infection memory such that there will be a more powerful response to future infections. Innate immune response is mostly provided by macrophages, dendritic cells, polymorphonuclear leukocytes, mast cells, natural killer cells, erythrocytes and platelets. The acquired immune response is provided by lymphocytes, the T (T cells) and B lymphocyte s (B cells). The lymphocytes are derived from hematopoietic stem cells (HSC) in the bone marrow. That form MLPs (myeloid-lymphoid progenitors). If the HSC and MLP stay in the bone marrow they form B cells and if they migrate (via blood) to the thymus they form T cells (see figure below). Initiation of immune response by the lymphocytes first requires recognition of the antigens and this is achieved by cell surface receptors called BCRs (B cell receptor) and TCRs (T cell receptor). These two receptors have great similarities and differences in their structure complexes, antigen recognition, cell activation and genetic recombination. A) STRUCTURE OF BCRs AND TCRs Both the BCR and TCR have great similarities and differences in the structure. They both exist as multi-chain complexes as seen in the diagrams below: i) Antigen recognition components In the figure above, section A shows the structure of a BCR. The BCR antigen recognition medium is an immunoglobulin (Ig) molecule (a transmembrane antibody). The antibody is modified via alternative splicing that adds a hydrophobic transmembrane domain and a short cytoplasmic domain (~3 aminoacids) at the C terminus of the immunoglobulin heavy chain (Wall Kuehl 1983). All naà ¯ve B cells only express both IgM and IgD classes of immunoglobulin but do switch to other classes upon activation by antigens (Goding, 1978). The antibody (figure 2C) is a highly specific Ig that can adopt any one of the 5 immunoglobulin isotopes, IgG, IgA, IgM, IgD and IgE. The antibody has 3 regions of which 2 regions (FAB) vary from antibody to antibody and bind to antigens and 1 region (FC) that binds to effector molecules. The antibody is composed of 2 light and 2 heavy chains held together by inter and intra disulphide bonds. The heavy chains depending on the Ig isotypes can be any one of ÃŽÂ ³, Ã⠀šÃ‚ µ, ÃŽÂ ±, ÃŽÂ ´ or Ɇº chains. The variable domains (VH and VL) bind to antigen and also bring about variability and antigen recognition specificity. This specificity is mainly due to the presence of 3 hypervariable regions (Complementary Determining Regions), namely CDR1, CDR2 and CDR3 in the variable regions. Similar to BCR, the antigen recognition medium in TCR is an immunoglobulin heterodimer made from ÃŽÂ ± and ÃŽÂ ² Ig chains (in most T cells) or ÃŽÂ ³ and ÃŽÂ ´ Ig chains. Unlike in BCRs where the IG can be of 5 types, in TCRs the Ig heterodimers are only of 2 types. The two Ig chains in TCRs are (also like BCRs) held together by intra and inter disulphide bonds. As seen in section C, each Ig chain folds into 2 domains, the variable and the constant domain. This folding greatly resembles the FAB region of the antibody in BCRs. Likewise antibodies, the ÃŽÂ ±ÃƒÅ½Ã‚ ² and ÃŽÂ ³ÃƒÅ½Ã‚ ´ heterodimers also have hypervariable regions (CDR1, CDR2 and CDR3) in variable domains. The variable regions in both BCRs and TCRs bring about specificity and diversity The BCR antibodies have a hinge joint (connecting FAB and FC) that makes the Ig molecule very flexible. Unlike antibodies, the flexibility of the TCR Ig molecule is very limited at the elbow region (junction of constant and variable region) (Degano et al, 1996). ii) ACCESSORY PROTEINS Both the BCR and TCR have very short cytoplasmic domains that restrict the binding of any signal transduction factors to the receptors. Due to this the receptors are unable to transducer signals into cells upon antigen recognition. Signal transduction is achieved via the accessory proteins. BCRs (figure 2 section A) accessory proteins consists of one or more dimmers of one each of Ig-ÃŽÂ ± and Ig-ÃŽÂ ² chains held together in the cell membrane by a pair of disulphide bonds. The cytoplasmic domains of these chains have phosphorylation sites called ITAMS. Unlike BCR accessory protein, the TCR accessory proteins (figure 2, section C) is composed of a complex know as CD3. It consists of 3 types of invariant chains, namely ÃŽÂ ³, ÃŽÂ ´ and Ɇº. A ÃŽÂ ³ or ÃŽÂ ´ chain couples up with one Ɇº chain (by formation of disulphide bonds) each to form two dimmers (ÃŽÂ ³Ãƒâ€°Ã¢â‚¬ º and ÃŽÂ ´Ãƒâ€°Ã¢â‚¬ º). In addition to this, a dimmer of 2 zeta (ÃŽÂ ¶) chains is also present. Together, these 3 dimers make up the CD3 complex. The ÃŽÂ ¶ chains have a much longer cytoplasmic tail than the ÃŽÂ ³, ÃŽÂ ´ and Ɇº chains and have 3 ITAMs as compared to one in the ÃŽÂ ³, ÃŽÂ ´ and Ɇº chains. Therefore for both BCR and TCR accessory proteins are dimmers that all contain ITAMs. B) GENERATION OF RECEPTOR DIVERSITY There are millions antigens and we need to produce millions of antibodies against them. However, we do not have millions of Ig genes so how are we able to produce all these different antibodies? The answer is antibodies are produced in developing B cells via genetic recombination of genes encoding the immunoglobulins (Hozumi and Tonegawa, 1976). The figure below shows the gene segments coding immunoglobulins. Figure legend: The human heavy chain locus as shown in the last row, consists of about 38-46 functional VH genes, 27 DH and 6 JH genes. The light chain can be either made of ÃŽÂ » or ÃŽÂ º chains. The ÃŽÂ » locus consists of about 30 functional V ÃŽÂ » genes and 5 J ÃŽÂ » genes each separated by a J segments. The Kappa locus has about 34-40 functional VÃŽÂ º genes and 5 JÃŽÂ º genes. The variable heavy chain region of the antibody is made from the joining of the V (variable), D (diversity) and J (joint) gene segments and the variable light chain (which can be either ÃŽÂ º or ÃŽÂ ») is formed from the joining of V and J segments only. A process called V(D)J recombination involves joining different gene segments and as a result bringing about antibody diversity. At the heavy chain locus, any one of the 27 D and 6 J gene segments are first joined together and then any one of 46 V gene segment is joined to this DJ segment. This rearranged DNA is then transcribed to form a primary mRNA. This mRNA then undergoes splicing to bring the VDJ segment close to the constant gene segment. Additional diversity is achieved as any 1 of the two types of light chains can be formed. Random insertion of nucleotides either side of D segments also creates N-nucleotide diversity. In total about 106 possible immunoglobulin gene combinations can be formed. This recombination process is driven by recombination signal sequences that flank the coding gene segments. Certain enzymes (RAG-1 and RAG-2) help mediate this somatic recombination process. The antibodies produce undergo a processs of clonal selectin where only the antibody specific to the antigen preferentially proliferates to make many antibodies. Binding affinity of BCR is greatly increased after antigen recognition where the variable regions of both heavy and light chain undergo somatic hypermutations. This is where point mutations are put in the variable regions of rapidly proliferating B cells. These mutations produce antibodies that may have good, moderate or good affinity for the antigens. The antibody with good affinity will have a selective advantage during clonal selection. The gene segements encoding TCR ÃŽÂ ² chain follow the similar V,D,J and C arrangement of BCRs. The recombination process involves of of the two DÃŽÂ ² genes rearranges next to one of JÃŽÂ ² genes. Then one of the ~50 V genes arranges next to the preformed DÃŽÂ ²JÃŽÂ ² genes. As seen , this is also similar to the B cells where a DJ segement forms first and then joins up with a V segment. There is also random insertion, just like in B cells, of nucleotides either side of D segments to create N-nucleotide diversity. Unlike in B cells, there is no somatic hypermutation in T cells after antigen recognition. If this occurs, the TCR will loose its ability to recognise MHC and the peptide it presents. C) ANTIGEN BINDING/RECOGNITION BCR and TCR have similar immunoglobulin antigen recognition receptors but the types of antigens they recognise are very different. BCR can recognise naÃÆ' ¯ve (as a whole) antigens and TCR can only recognise a single antigen peptide sequence presented onto cell surfaces by MHC (Major histocompatibility complex) molecules. The antigens recognised by B cells are naÃÆ' ¯ve and therefore the antibody in BCR mostly recognise discontinuous epitopes on the antigen and antigens recognised by the TCR is in form of linear peptide sequences and therefore they mostly recognise continuous or linear epitopes. Antigen recognition by BCR is very simple where the antibody variable region simply recognises specific epitopes on antigen and bind to it. The BCR can recognise 3 types of antigens, Type 1 thymus independent antigens (where bacterial lipoproteins can bind to mitogenic bypass molecules on B cells surface and this allows non-specific antigen B cell activation), Type 2 thymus independent antigens (appiles to antigens that have well spaced and repetitive polysaccharides that bind to multiple antibodies in BCR and activate the B cell) and Thymus dependent antigens (require helper T cells). Thymus dependent antigens when bind to TCR, instead of causing activation normally cause anergy. Due to this, once the binding has occurred, the whole antigen+TCR comples is endocytosed, the antigen is hydrolysed by enzymes and processed to small linear peptides and then presented onto the B cell surface via MHC2 molecules. Helper T cells then recognise this peptide-MHC complex. B cells have loads of C D40 on their surface that binds to CD40L present on Th helper cells. In response to this Th cells secrete IL-4, 5, 6 that also help activate other costimulatory molecules in the BCR coreceptor complex. All these events provide costimulation of the B cells and it is activated. ÃŽÂ ±ÃƒÅ½Ã‚ ² heterodimer TCRs in comparison can recognise any type of antigen that is processed and presented as a single peptide on MHC1 on target cells and MHC2 on B cells, macrophages and dendritic cells (all professional antigen presenting cells). The non-covalent forces that help TCR bind to the peptide-MHC complex are similar to the forces that enable the antibody bond to the antigen i.e. noncovalent. Unlike BCR that only have to recognise epitopes on antigens, the TCR has to both recognise the presence of both MHC molecule and antigen peptide. The TCR VÃŽÂ ± (variable alpha region) overlays ÃŽÂ ±2 helix of MHC1 or ÃŽÂ ²1 helix of MHC2 and the VÃŽÂ ² domain overlays ÃŽÂ ±1 helix in both MHC1/2. The CDR1 and CDR2 bind to ÃŽÂ ± helices of MHC and the CDR3 (which is more variable), binds to the antigen peptide on MHC. This concept is summarised in the picture below: Figure legend: The picture shows how the TCR variable complementarity determining regions (CDR) which are the binding sites interact with peptide-MHC complex. The CDR1 and CDR2 bind to the MHC alpha helices and CFR3 binds to the peptide. The ÃŽÂ ³ÃƒÅ½Ã‚ ´ TCRs are more similar to BCR antibody as they can recognise naÃÆ' ¯ve antigens without the requirement of processed antigen presentation. Another similarity of BCR and ÃŽÂ ³ÃƒÅ½Ã‚ ´ TCRs is that in the antibodies of BCRs, the CDR3 regions on heavy chain are shorter than the CDR3 in heavy chains and also the same in ÃŽÂ ³ÃƒÅ½Ã‚ ´ TCRs is seen where the ÃŽÂ ³ are shorter than the ÃŽÂ ´ CD3. COSTIMULATIONS Both lymphocytes do not get activated (but undergo anergy) once they recognise and bind to an antigen. They require costimulatory signals that will eventually lead to the activation of the lymphocytes. The B cells have BCR co receptor complex consisting of CD19 and CD21 (complement receptor), CD81 and LEU13 (interferon induced transmembrane protein 1). All these molecules are stimulated in presence of interferons and complements that give a costimulatory signal to B cells and activate it when it has recognised an antigen. The precise details of how these costimulatory molecules stimulate B cell signalling are still under investigation. In contrast to the 4 main costimulatory molecules in B cells, the primary costimulatory molecule in T cells is CD28 (figure besides) The binding of peptide-MHC to TCR causes up-regulation of certain molecules (e.g. CD28). T cells, like B cells can be costimulated by either cytokines or costimulatory molecule interactions. APC have surface molecules such as the B7.1 and B7.2 (or the CD80 and CD86) that recognise and bind to a molecule on the surface of the T cells called CD28 found on CD. This interacting provides co stimulation. The CTLA4 molecule is highly expressed after proliferation of the T cells. Once it binds to B7, instead of co stimulating T cells, it turns the T cells off. This is helpful in preventing excessive immune responses. No such regulatory mechanism is seen in B cells. A unique feature of T cells is that they have co receptors (CD4 and CD8) that help recognise the MHC molecules. CD4 molecules act as co receptors for MHC2 and are found on helper T cells and CD8 molecules present on cytotoxic T cells help recognise MHC1 molecules. ACTIVATION OF B AND T CELLS The activation of B and T cells following antigen recognition is somehow similar as it involves the phosphorylation of the ITAMS of accessory proteins. In B cells, antigen binding and co stimulation recruits the BCR+antigen to lipid rafts that brings protein tyrosine kinase Lyn close to the ITAMs of the cytoplasmic tails of the BCR associated proteins. Lyn phosphorylates ITAMs and triggers a signal cascade that results in increase of cytoplasmic calcium levels that activate transcription factors that control the entry of B cells into cell cycle. Eventually activate the B cells which then form plasma cells (that make loads of clones of antibodies to the antigen) and memory cells that will help manage subsequent infections. The initial proliferation of the activated B cell is accompanied by somatic hypermutation of the rearranged antibody variable genes that lead to the production of antibodies that may have poor, moderate or good binding capacity to the antigen. The good binding antib odies will be preferentially selected during clonal selection and they will further undergo proliferation to produce plasma and memory cells. A similar situation also occurs in T cells where there is activation of lipid rafts that bring the zeta chain ITAMS close to Lck (a protein tyrosine kinase) that phosphorylates the ITAMs and therefore create opportunity for other factors to bind to it and eventually cause mobilization of calcium that causes proliferation of T cell into Helper T cells, Regulatory T cells and Cytotoxic T cells.

Swarovski Branding Strategies & Products

SWAROVSKI. Branding for luxury goods Group G. Members: Truong Anh Bao Nguyen Yunkyung Choo Lilit Nagapetyan CONTENT: 1 History 2 Concept 3 Brand Strategy 4 Marketing Mix 5 Positioning 6 Services 7 Target Consumer 8 CBBE Pyramid 9 SWOT analysis 10 Competitors 11 Recommendation 12 References 1. HISTORY: 1882 Daniel Swarovski (1862-1956) invents a revolutionary machine that allows crystals to be cut more precisely than with existing manual methods. 1895 Daniel Swarovski founds the company in Wattens, Tyrol, with the vision of bringing joy to people through crystal.His guiding principle is still followed by the company today: â€Å"To constantly improve what is good. † 1949 SWAROVSKI OPTIK is founded, and goes on to become a leading manufacturer of precision optical instruments for hunting and nature observation (binoculars, telescopes, rifle scopes, range finders, and night vision and optronic devices). 1956 The first Swarovski crystals for chandeliers and lighting are launched, and in 1977 are registered under the STRASS Swarovski Crystal name.Today they adorn classical chandeliers, suche as those in the Palace of Versailles and the Metropolitan Opera, New York, as well as more modern lights. Swarovski starts to manufacture precision-cut gemstones. 2002 Swarovski develops Crystal Fabric: countless tiny crystals create a delicate shimmer over a variety of materials. 2003 In conjuction with leading fashion and jewelry designers, â€Å"Runway Rocks† is founded – a collection of unique jewelry pieces featuring crystal for the catwalk, showcasing design and innovation 2009 At the â€Å"Baselword† watch and jewerly exhibition, Swarovski launches its first watch collection. . CONCEPT More than a century ago, Daniel Swarovski, its founder, once said â€Å"A diamond of everyone† that sparkles everyone's lives, Swarovski today has been thriving for the best to serve its customers, being one of the finest crystal producers in today’ s global industry. Mission Through the mastery of the poetry of precision we continue to be market leader, driving force and reliable partner within our industry to meet people’s desire for adornment and delight since 1895 as * manufacturer, marketer, and retailer of premium jewelry and customer products ranging from decorative objects to lighting and accessories. manufacturer and marketer of premium jewelry stones for customers. * Swarovski provides high quality products and services and anticipate, fulfill and exceed consumers’ desires and customers’ needs. * Swarovski offers our colleagues and teams fulfilling challenges that inspire them to be innovative and creative. 3. BRAND STRATEGY: Not so long ago Swarovski was not very famous and didn't have any branding strategies. ‘For a long time Swarovski didn't â€Å"brand† its product. And back then it wasn't really about branding as it is nowadays.We had to develop a hard-core communications strateg y – a PR strategy – which we pitched to the editors and designers. ‘ said Nadja Swarovski, Swarovksi Vice president of International Communications. Today, Swarovski is almost omnipresent everywhere from fashion awards, catwalk shows, film premieres; its crystals are used by fashion designers from the edgiest Central Saint Martins graduate to the hallowed ateliers of Armani, Dior and Chanel. This is where the real success of Swarovski lies: by aligning the company with the most avant-garde young fashion talent.When the hottest British designers of the time, Alexander McQueen, Philip Treacy and Julien Macdonald used Swarovski crystal mesh – a fine fishnet gauze studded with tiny crystals – in his catwalk show, it immediately changed people's perception of the brand. ‘The McQueen show provided a visual that was very different to the standard idea people had of us,' Nadja says. The company then began to re-establish the company's links with the f ashion world, sponsoring younger designers who are in need of capital to make their label take off.Nearly ten years later this winning formula is still producing results. Designers, no matter how edgy or cool, all seem to want to use Swarovski crystals and accept sponsorship. ‘If you're a young designer starting a collection, it's great to have crystal in there because it makes it more haute couture,' Nadja says. ‘It adds elegance, a seriousness, credibility. In fashion, if you have something to prove, then couture, or at least craftsmanship, is the right track. â€Å" Not only enraptured the fashion world, Swarovski also moved on to the film industry.Some of Swarovski's most high-profile projects have been Moulin Rouge, where the sets and costumes groaned under the weight of Swarovski crystals, Titanic and the recent Bond films. During Oscar season Nadja sets up camp in Los Angeles, and stylists can browse the Swarovski jewellery, shoes and handbags with a view to dres sing their clients on the red carpet. These days Swarovski crystals adorn everything from iPods and mobile phones to Maria Sharapova's new Nike tennis dress. They even covered, whisper it, Jordan's wedding dress. 4.Marketing Mix Products: The Swarovski Crystal range includes crystal glass sculptures and miniatures, jewelry and couture, home decor, and chandeliers. They're best known for imitating â€Å"colored germs† All sculptures are marked with a logo. The original Swarovski logo was an edelweiss flower, which was finally replaced with the current swan logo in 1988 Subsidiary companies: Swarovski Fashion accessories and crystal-based ornaments. Daniel Swarovski Jewelry, handbags, accessories and interior design objects. Swarovski Optik Optics.Atelier Swarovski Fashion and jewellery designers. Swarovski Crystal Palace Avant-garde lighting and design (chandeliers etc. ). Swarovski Elements Crystal designs. Swarovski Gemstone Business Gemstone designs. Swarovski Lighting Swar ovski finished lighting products and solutions with crystal for architecture. Tyrolit A bonded grinding and dressing tools company. Swareflex A road safety products specialist. Schonbek A crystal chandelier manufacturer. Touchstone Crystal Swarovski's direct sales company for ready-made jewelry PlaceAll boutiques are beautifully set up and conveniently located in the fashion mall/heart of big 19 metropolitan cities from Dubai, Madrid, Hongkong to New York City over 20 countries in North America, Europe, Asia and Australia. Also online website is available with convenient shipping services Price 100$ to $42,800/item Promotion Promotion is via press, social network (Twitter, Facebook, Youtube) as well as via traditional and on-going sponsorship for young talented artists/ designers to use Swarovski crystals in any kinds of their designs in fashion show, film awards, etc.Therefore, the best season to promote Swarovski crystals is during the fashion week (twice a year- January to March and from September to October) and movie/film award ceremonies seasons (October to February) 5. Positioning Swarovski is positioning itself as a luxury fashion by creating a customer experience to let people know Swarovski’s brand values of innovation and modernity. They would like to position themselves as creating premium, high-quality products for the consumers. They are trying to be a market leader in the luxury jewelry industry by innovating continuously.Recently, Swarovski hired Yellowdoor as retained agency, which will focus on positioning as an affordable luxury brand. * Luxury fashion brand * Market leader in the luxurious crystal industry * Multi-faceted business * Affordable luxury brand 6. Service * Collection of rings, pins, earrings, pendants, necklaces, hair jewels, cuff links, charms, brooches, bracelets, bangles and gifts. * Large variety of color options * Vast selection of gifts available for fewer than $100 * Magazine and group for Swarovski enthusiasts. * Free shipping standard on orders more than $95 * Customized gift messages Free gift wrapping and bag * Scheduled delivery * Right to return (2 week return policy) * Product warranty * After sales services 7. Target Customer Swarovski's target customer is â€Å"every female† from 17 to 71 in terms either of buying or gift receiving because they offer diverse products to such a broad range of people. Even though each group (tweens, generations X,Y and Baby Boomers) has its own needs and values, Swarovski meets most of them. Swarovski Jewellery bases most of its market on women of age 25 and above by creating bridal, business, classical and sophisticated collections.It also include men accessories such as bracelets and watches, and of course kids over 6 years old. Also, one of the biggest Swarovski's market target today is fashion industry. Swarovski crystals are affixed to everything from Victoria's Secret bra sets and Kawasaki motorcycles, to the clothing collections of such as Dolce ; Gabbanna and avant-garde vintage revisionists Imitation of Christ. 1) Women collection of * Sophisticated * Business * Classical * Bridal collections 2) Men MEN’s collection * Rings * Bracelets * Necklaces 3) Kids * Disney collection Hello Kitty collection 4) Other Industries: Swarovski crystals are affixed to everything. * Victoria's Secret bra sets * Kawasaki motorcycles * Clothing collections * DJ Headphones 8. CBBE Pyramid Resonance: HIGH LOYALTY Brand resonance is high due to active loyalty of customers Consumer Judgments: GLOBAL BRAND WITH RICH HERITAGE Perfect and innovative crystal products High quality production Consumer Feeling: PRESTIGIOUS ELEGANCE Prestigious, elegant, fragile, dedicated, timeless Humble and sophisticated Feels like a fairy Brand Performance: EXCELLENT SERVICESExtremely durable, serviceable Offers timeless, high quality crystal products Innovative product lines: Optik gears, etc. Brand Imagery: GLAMOROUS Glamorous luxurious crystal pro ducts Women/young ladies with high income, high taste of elegant sophisticated jewelries Brand Salience: HIGH DEPTH & BREADTH Extremely high recognition on the crystal market Most known for its crystal jewelries product line If it's crystal, it’s nothing else but Swarovski! 9. SWOT Analysis: Strengths Strong brand identity and high company reputation World widely recognized market leaderCheap materials, high added value products Craftsmanship (durable quality) Online store New techniques Perfection High quality After sales services Weaknesses Expensive production in Austria Repetitive design Opportunities Diversity of customers of all ages New markets in Latin America ; emerging markets in Asia Various product lines through collaboration Unlimited design according to crystal cutting Threats Cheaper competitors Imitators/substitutes Relies on disposable income of consumers Slowdown in market growth Cheaper artificial crystal 0. Competitors Price: Cartier – Swarovski â⠂¬â€œ Pandora – Preciosa Reputation in jewelry market: Cartier – Swarovski – Pandora – Preciosa Variety of jewelry products: Pandora – Swarovski – Cartier – Preciosa Quality of crystal: Swarovski – Cartier – Preciosa – Pandora 11. Recommendation †¢Product Strategy Swarovski should expand its business in the jewelry market, having more product lines as well as jewelry designs since it's already has a reputation in the crystal industry: Extend their product lines (perfumes, watches, Home electronics , etc. Extend their jewelries products (leather bracelet, wedding ring, etc. ) Focus on jewelry customization †¢Place: Establish stores in Latin America and expand business in emerging markets †¢Pricing No changes †¢Promotion No changes 12. REFERENCE: http://www. swarovski. com/ http://www. brand. swarovski. com/Content. Node/home. fr. html#/en/aboutus/ourevolution http://www. brand. swarovski. com/Co ntent. Node/home. fr. html#/en/aboutus/spirit http://swarovskijewrley. blogspot. fr/2010/09/week-4-marketing-environment. html http://online-jewelry-review. optenreviews. com/swarovski-review. html http://www. brandchannel. com/ http://www. prweek. com/uk/news/890882/Swarovski-hires-Yellowdoor/? DCMP=ILC-SEARCH http://fashion. telegraph. co. uk/news-features/TMG3361538/Swarovski-the-glitz-spirit. html http://www. crystalfanaticsclub. com/about_swarovski. php http://globalfashionanalytics. com/ritejl/brending/157-marketingovaja-strategija-v-ljuksovom-segmente. htm http://news. naver. com/main/read. nhn? mode=LSD&mid=sec&sid1=101&oid=001&aid=0005922714

Call of the Wild- Chapters 4 5 6

Chapter 4 What happens when Chris/ Alex drives the Datsun into the Lake Mead National Recreation Area? A flash flood comes and floods the engine of his Datsun. He is frustrated, and in his frustration he kills the battery trying to get it restarted. Rather than go to the local authorities (He decides he shouldn't due to his expired registration and license) he abandons the car with a note saying anyone who can get it out can have it. What do his parents think? Alex’s parents are very worried about him. They become even more worried when they realize that he told the post office to hold all of the letters they sent him until a certain date.Where does Chris/Alex travel in this chapter and what is the time line (dates and locations) July 9th 1990- Datson is flooded is flooded at Lake Mead July 10th 1990- Chris hikes toward N California August 1990- He gets a ride from/meets Crazy Ernie Chapter 5. What did the people of Bullhead City think of Chris/Alex? He is remembered as a stra nge but nice person. The employees at the McDonald’s where he worked said he worked at a slow pace, which contradicts what was said about his work ethic earlier in the novel. They said he needed to bathe more and one lady feels he quit because she told him so.Describe the slabs in your own words. The slabs are an abandoned military base that has become home to a lot of drifters. What do we learn about Chris/Alex in this chapter? Is your view of him changing? In this chapter we learn that Chris’s work ethic is starting to disappear because the former employees say that they felt he worked slow. My opinion is not really changing any of him because he still is very odd and obviously he is not all right. 4. Trace the timeline in this chapter. May 1991- Departs Las Vegas July/August 1991- Oregon coastSeptember 1991- Hitchhiked down highway 101 to Bullhead city Several months later- moved to the slabs Chapter 6. What changes do you notice in the narrative stance of chapter 6 ? Chapter 6 isn’t really about chris, its more about the people he met and the lives he affected. Why did they send Krakauer a letter from Ronald Franz? Because Franz wanted a copy of the magazine article which featured Chris and felt very attached to him. In Chris/Alex's letter to Ron Franz he reveals his philosophy of life. This is important for an understanding of what happened to him. Summarize his ideas in your won words. If you want to get more out of life, Ron, you must lose your inclination for monotonous security and adopt a helter-skelter style of life that will first appear to be crazy† 57. This is important because for the first time in words he is sort of describing the lifestyle that he is trying to live. He put the ideolism that he is living by into a phrase. What did Krakauer learn when he visited Franz? He learned that Franz was a very physically large men who did not show his age. He had always been praying for Chris to walk back down the road. When he learned that Chris had died, â€Å"{he} became an athiest† because his prayers were not answered†

Female Prostitution Annotated Bibliography Examples

Tyler, K., Hoyt, D., and Whitbeck. The Effects of Early Sexual Abuse on Later Sexual Victimization among Female Homeless and Runaway Adolescents. Journal of international violence 15 (3) March 2000 235-250. The authors Tyler, Hoyt, and Whitbeck wrote this article focused on the risks magnification and victimization approaches. They used path analysis to examine the influence of child and youth sexual abuse. The study involved investigating behaviors and background information on some homeless females and adolescents in different Midwest states. The authors found out that the child and youth sexual abuse have an affirmative direct influence on sexual persecution of adolescence on the streets. They also confirmed that child and youth prostitution raises the possibilities of later sexual persecution indirectly by raising the amount of time at risk, abnormal peer relations, and occurrence of survival sex. The article also discusses on the repercussion of some of the child and youth behaviors that they will carry on to later lives. The authors further indicated that young females who leave dysfunctional and muddled homes subsequently are described by continued abused on negative growth paths once they are in the streets. The social context of adolescence on the run or streets puts their lives in close range to possible criminals and exposes them to risks of being jailed for street misconducts. The mixture of a negative progression path and increased risks of streets and its surrounding raises these children and youth’s chances of being sexually molested. Ferguson, K. Exploring Family Environment Characteristics and Multiple Abuse Experiences among Homeless Youth. Journal of interpersonal violence. 24 (2009) 1875-1891. The author Mr. Ferguson (2009) explores the lives of street females to establish their views on the family environment illustrations and abuse experiences. He used a constant comparative technique to investigate records from in depth analysis with the youth participants. The main objective of the article is to find different from of family characteristics such as instability, desertion, and caregiver abuse. Other discussed issues include child and youth rejection, intrafamilial abuse, caregiver abuse, and condemnation by caregivers. The author applied the grounded theory to discuss on the outcomes and create a practical hypothesis to control future studies of various maltreatments among the children and adolescences. The author also discussed on various abuse experiences that the youth and children underwent before they decide to seek refuge in the streets. Some of the experiences that the adolescence went through include emotional, physical, verbal, and sexual, discrimination in addition to experiencing familial abuse. The author also examined the factors that catalyzed verbal exchanges and this are substance like drugs and alcohol. He also notes that it is essential to study family environments and abuse experiences of the street children since the main factors causing adolescence to seek refuge in streets and prostitution are family dysfunction and maltreatment. Philpot, C., Harcourt, C., and Edwards, J. A survey of female prostitutes at risk of HIV infection and other sexually transmissible diseases. An international peer-reviewed journal for health professionals and researchers in all areas of sexual health. 67 (1991) 384-388. The authors in this article established risk factors for the infection of Human Immunodeficiency Virus (HIV), in addition to injecting drug use (IDU), sexual character, and other sexually transmitted diseases in female prostitutes. The authors carried out a detailed questionnaire on adolescence women and tested the results to determine extend of risks to which female prostitute are involved. For close analysis of this article, the author first defined the term prostitution as a person who offers sexual services for money or other material benefits. Then they narrowed it down to adolescence sexual abuse. In spite of the government’s effort to fight for protection of child’s rights, there are some who still do not see the need to practice that and they are involved in persuading children to participate in street prostitution thus exposing them to a higher risks of contracting the HIV diseases. The authors confirmed that although there is change in character of some adolescence, there still is a quite number practicing prostitution. They therefore, suggest measures that can be used by females to protect themselves against the infections. The measures include extensive use of barrier mechanisms of contraceptives, campaign to prevent the sharing of intravenous needles, close supervision of health of prostitutes, and they finally proposed the use of scientific study on both the practicing male and females to determine the rate at which the disease is spreading. Graham, Nanette, Wish, Eric D. Drug use among female arrestees: Onset, patterns, and relationships to prostitution. Journal of Drug Issues, 00220426, Winter/Spring94, Vol. 24, Issue 1/2 This article deals with the lives of female arrestees who found rooming the streets with no specific jobs. The authors illustrated various issues affecting adolescence street women. The main factors discussed by the authors are behavioral change and use of drugs. They described in details the information they caught from their interviews with the adolescence and noted that most street women were exposed to several character and psychological problems as well as drug abuse and prostitution. Consequently, most arrestees were high school dropouts and they originated from broken families. The article also deals with various social programs that should be initiated to address child and youth issues living in the streets. The article also found out that most street children follow the footsteps of the mothers. The authors therefore, suggest that street children should be given medical, psychological, and social attention since they are ignorant of what is happening to them. The authors also confirm that there is close or direct relation between street children’s character and their early lives. The on set of drug abuse is illustrated by the psychological trauma that the children underwent before they finally find themselves in the streets practicing prostitution and other related crimes. Bullough, Bonnie, Bullough, Vern, L. Female prostitution: Current research and changing interpretations. Annual Review of Sex Research, 10532528, 1996, Vol. 7 Any form of prostitution mainly targets the young females to exploit their innocents. The authors of this article confirm the allegation and continued to discuss on what major challenges are affecting young women to join prostitution. They analyze the trends that prostitution have undergone and found out that the details and information have changed over the years. Prostitution used to be practiced by the elder women but currently that have changed and children and youth dominates the scene. The authors also illustrated that young women have been forced to work as sex slaves and despite their efforts; they are still underpaid a thing that makes them live in the streets and lead a miserable life. The change in trends has other consequences that make the young women continue living in the streets. Some have even grouped themselves in groups to protect their interests and a team. The authors conclude by discussing the various reasons that tempt females to join the sex industry. References Bullough, Bonnie, Bullough, Vern, L. Female prostitution: Current research and changing interpretations. Annual Review of Sex Research, 10532528, 1996, Vol. 7 Graham, Nanette, Wish, Eric D. Drug use among female arrestees: Onset, patterns, and relationships to prostitution. Journal of Drug Issues, 00220426, Winter/Spring94, Vol. 24, Issue 1/2 Philpot, C., Harcourt, C., and Edwards, J. A survey of female prostitutes at risk of HIV infection and other sexually transmissible diseases. An international peer-reviewed journal for health professionals and researchers in all areas of sexual health. 67 (1991) 384-388. Ferguson, K. Exploring Family Environment Characteristics and Multiple Abuse Experiences among Homeless Youth. Journal of interpersonal violence. 24 (2009) 1875-1891. Tyler, K., Hoyt, D., and Whitbeck. The Effects of Early Sexual Abuse on Later Sexual Victimization among Female Homeless and Runaway Adolescents. Journal of international violence 15 (3) March 2000 235-250.